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KMID : 0379519990150030339
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한국독성학회지
1999 Volume.15 No. 3 p.339 ~ p.344
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Antitomor Evaluation of Methyl Gallate Isolated from Acer Ginnala Max
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Han Du-Seok
Han Seong-Soo
Lee Jung-Ock
Rim Yo-Sup
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Abstract
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In order to develop antitumor agent which has weak side effects and strong antitumor activcytotoxicity and antitumor effects of methyl gallate (MG) was evaluated by MTT and SRB assay of colorlmetric methods on the cultured NIH 3T3 fibroblasts and human oral epitheloid carcinoma cells (KB cells). NIH 3T3 fibroblasts were cultured in EMEM and KB cells were cultured in RPMI 1640 media. After incubation for 24 hrs, the cells were treated with MG by dose dependent manner (1, 25, 50, 100 μM), incubation 48 hrs, MTT and SRB quantity was measured by ELISA reader (Spectra Max 250, USA). The morphological changes of KB cells were observed by inverted microscope. IC50 by SRB assay of human lung tumor cells, human ovarian tumor cells, human melanoma tumor cells, human colon tumor cells and human CNS tumor cells, when MG was treated at them, was 69.80 μM, 44.90 μM, 22.12 μM, 29.24 μM and 59.29 μM, respectively. IC50 by SRB assay was 8701.23 μM in NIH 3T3 cells and 168.81 μM in KB cells treated with MG. Morphological changes of NIH 3T3 cells treated with MG were weak, but those of KB cells treated with MG were severe.
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KEYWORD
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Antitumor activity, Cytotoxicity, MTT and SRB assay, Morphological changes
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